Plateforme HTS Core Facility
The High-Throughput Screening Core Facility (HTS Core Facility) of the Institut de la Vision is a core facility aiming at developing new screening approaches linked to academic research and private companies.
Presentation
The HTS Core Facility is dedicated to large and very large libraries screening such as small compounds, natural compounds, cDNA, and siRNA collections using original and highly valued biological models. As key points, the extensive sterility and the speed of robotic processes allow us to safely run large scale assays involving cell lines, primary cells, human stemcells-derived progenitors (hESC, iPSC).
We closely work with our public and private partners to develop clear and feasible screening projects from the design of the screening “cascade” to the plan for hit list validation. Once the screening process is designed, the HTS Core Facility carries out the development of the related assays fulfilling the robotic standards and constraints. We obviously take in charge the complete automation of the screening process and, finally, the screening of the in-house or external chemical libraries.
Expertise
The cell culture platform at the Institute of Vision specializes in the following areas of expertise:
- high throughput screening and medium throughput screening
- lab automation
- library management
- assay development
Equipment
- 2 sterile automates (Agilent custom Biocel1800 & Bravo workstation)
- 1 2D automated microscope (Thermo-Cellomics)
- 1 automated confocal microscope (Yokogawa) and a wide panel of algorithms for the quantification of complex biological events, (protein translocations, cell differentiations, biomarker expression, cell migration).
As maximums, we able to carry out from 85,000 (384-well plates) to 362,000 tests (1536-well plates) in a single operation, and in sterile conditions.